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Ways of radioactive materials saving in the Czech Republic and in the world
Polák, Luboš ; Šen, Hugo (referee) ; Nerud, Pavel (advisor)
Management of spent nuclear fuel and radioactive waste are many. The aim of this thesis was to summarize the attitude of the Czech Republic and the other major countries on this issue. First is given the classification of radioactive waste according to various criteria. Consequently, it is defined and described the fuel cycle, as the largest producer of all types of radioactive waste. Then is analyzed approach of the Czech Republic to the management of all type radioactive waste and the attitude to deep geological repository. At the end are some, in this respect, major countries and their attitude to deal with spent nuclear fuel, radioactive waste and the method of final disposal.
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The study of occurrence, collection and disposal of hazardous waste
Wolf, Adam ; Benešová, Libuše (advisor) ; Zimová, Magdalena (referee)
This bachelor thesis provides an overview about the hazardous waste. At the beginning there will be discussed the legislative measures related to hazardous waste management and international cooperation. Following three chapters are dedicated to these hazardous wastes: asbestos waste, sludge from wastewater treatment and ashes from incineration waste. Today forbidden material asbestos occurs in insulation of the old public and industrial buildings. It is essential to prevent release of asbestos fibres. Further currently was proposed several promising processes for treatment of sewage sludge and ashes from incineration waste that management in the Czech Republic overlooks. Presented methods emphasize degradation of hazardous properties from the concerned waste. Thesis shows current occurrence of waste in environment. Main objective is assessment of potential risks for human health or environmental components, consequent from hazardous waste and draw implications for the most effective treatment. A conclusion of literature research summarized advice for treatment of these waste. Key words: hazardous waste, hazardous properties, asbestos containing waste, sludge from wastewater treatment, sewage sludge, waste incineration, bottom and fly ash, vitrification, heavy metals.
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Study of changes on spindle and chromosomes vitrified and in vitro maturated human oocytes
Holubová, Kateřina ; Kratochvílová, Irena (advisor) ; Libusová, Lenka (referee)
The aim of this work is the comparison of cryoprotection methods for human oocytes regarding their survival rate and subsequent success in in vitro fertilization. This work showed the effect of oocyte maturation characterized by the state of the meiotic spindle, chromosomes and polar body on the success of in vitro fertilization of standard (fresh) and vitrified oocytes. The acquired knowledge will lead to a better understanding of the relationships between the state of oocytes and specific in vitro fertilization and cryoprotection procedures. The final, application-interesting goal will be to propose the optimization of methods for working with oocytes so that the probability of their success in the IVF cycle is as high as possible.
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Electron cryo-microscopy techniques in biological research and nanotechnologies
Mistríková, Veronika ; Bednár, Jan (advisor) ; Nebesářová, Jana (referee) ; Benada, Oldřich (referee)
Preparation of biological samples for transmission electron microscopy is not a trivial task. The samples must withstand a vacuum environment present inside a microscope, and it is often necessary to use non-physiological procedures for their processing. These procedures usually involve aldehyde-based fixation, replacing water with alcohol (i.e. dehydration/substitution), and embedding into a resin, which creates support for the subsequent preparation of thin sections that can be placed into the microscope. In the last decade, the method of cryo-fixation (vitrification) using ultra-fast high-pressure freezing followed by freeze substitution and low-temperature resin embedding gained a dominant position in the cell biology research. In this way, a range of biological samples with a thicknesses up to several hundreds of micrometers was successfully vitrified to a state that was closely related to their in vivo structures. The cryo-fixation of isolated biological objects (with a limited thickness up to several micrometers) is possible in a thin layer of vitrified water by plunge freezing at ambient pressure. In combination with electron cryo-microscopy, this method has become the most effective and fundamental principle for the high-resolution studies and image analysis of fully hydrated samples...
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Study of changes on spindle and chromosomes vitrified and in vitro maturated human oocytes
Holubová, Kateřina ; Kratochvílová, Irena (advisor) ; Libusová, Lenka (referee)
The aim of this work is the comparison of cryoprotection methods for human oocytes regarding their survival rate and subsequent success in in vitro fertilization. This work showed the effect of oocyte maturation characterized by the state of the meiotic spindle, chromosomes and polar body on the success of in vitro fertilization of standard (fresh) and vitrified oocytes. The acquired knowledge will lead to a better understanding of the relationships between the state of oocytes and specific in vitro fertilization and cryoprotection procedures. The final, application-interesting goal will be to propose the optimization of methods for working with oocytes so that the probability of their success in the IVF cycle is as high as possible.
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Implementation of novel methods of tissue preparation and storage for transplantation purposes in ophthalmology
Rybičková, Ivana ; Jirsová, Kateřina (advisor) ; Vlková, Eva (referee) ; Čejková, Jitka (referee)
Introduction: Recent advances in posterior lamellar keratoplasty necessitated the preparation of posterior corneal lamellae even in the Czech Republic. The aim of this work was to introduce and standardize a novel method of manual preparation of corneal lamellae for Descemet Membrane Endothelial Keratoplasty with a Stromal rim (DMEK-S) in an ocular tissue bank. After setting the criteria for endothelial quality, we obtained a licence to provide the tissues for transplantation purposes. The obtained results were analysed after two years. Furthermore, a novel lamellar insertion technique using a cartridge was assessed. The potentials for the long-term storage of posterior corneal lamellae by the vitrification in liquid ethane was studied using human amniotic membrane as a model tissue. Material and Methods: Corneoscleral buttons stored in tissue cultures were used to prepare lamellae consisting of a central zone of endothelium and Descemet membrane supported by a stromal rim at the periphery. The manual preparation was performed on an artificial anterior chamber using the big bubble technique. Endothelial quality was assessed before storage, before and immediately after preparation as well as after 2 days of storage at 31řC. A group of 12 corneas with a live endothelial cell density ≥ 2500 cells/mm2...
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The study of occurrence, collection and disposal of hazardous waste
Wolf, Adam ; Benešová, Libuše (advisor) ; Zimová, Magdalena (referee)
This bachelor thesis provides an overview about the hazardous waste. At the beginning there will be discussed the legislative measures related to hazardous waste management and international cooperation. Following three chapters are dedicated to these hazardous wastes: asbestos waste, sludge from wastewater treatment and ashes from incineration waste. Today forbidden material asbestos occurs in insulation of the old public and industrial buildings. It is essential to prevent release of asbestos fibres. Further currently was proposed several promising processes for treatment of sewage sludge and ashes from incineration waste that management in the Czech Republic overlooks. Presented methods emphasize degradation of hazardous properties from the concerned waste. Thesis shows current occurrence of waste in environment. Main objective is assessment of potential risks for human health or environmental components, consequent from hazardous waste and draw implications for the most effective treatment. A conclusion of literature research summarized advice for treatment of these waste. Key words: hazardous waste, hazardous properties, asbestos containing waste, sludge from wastewater treatment, sewage sludge, waste incineration, bottom and fly ash, vitrification, heavy metals.
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Electron cryo-microscopy techniques in biological research and nanotechnologies
Mistríková, Veronika ; Bednár, Jan (advisor) ; Nebesářová, Jana (referee) ; Benada, Oldřich (referee)
Preparation of biological samples for transmission electron microscopy is not a trivial task. The samples must withstand a vacuum environment present inside a microscope, and it is often necessary to use non-physiological procedures for their processing. These procedures usually involve aldehyde-based fixation, replacing water with alcohol (i.e. dehydration/substitution), and embedding into a resin, which creates support for the subsequent preparation of thin sections that can be placed into the microscope. In the last decade, the method of cryo-fixation (vitrification) using ultra-fast high-pressure freezing followed by freeze substitution and low-temperature resin embedding gained a dominant position in the cell biology research. In this way, a range of biological samples with a thicknesses up to several hundreds of micrometers was successfully vitrified to a state that was closely related to their in vivo structures. The cryo-fixation of isolated biological objects (with a limited thickness up to several micrometers) is possible in a thin layer of vitrified water by plunge freezing at ambient pressure. In combination with electron cryo-microscopy, this method has become the most effective and fundamental principle for the high-resolution studies and image analysis of fully hydrated samples...
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Ways of radioactive materials saving in the Czech Republic and in the world
Polák, Luboš ; Šen, Hugo (referee) ; Nerud, Pavel (advisor)
Management of spent nuclear fuel and radioactive waste are many. The aim of this thesis was to summarize the attitude of the Czech Republic and the other major countries on this issue. First is given the classification of radioactive waste according to various criteria. Consequently, it is defined and described the fuel cycle, as the largest producer of all types of radioactive waste. Then is analyzed approach of the Czech Republic to the management of all type radioactive waste and the attitude to deep geological repository. At the end are some, in this respect, major countries and their attitude to deal with spent nuclear fuel, radioactive waste and the method of final disposal.
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